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Archive | October, 2012

The peace in qPCR

It’s no secret that qPCR is my bag; it’s been almost my sole focus since the summer of 2007.  I am not, by any stretch of the imagination, an expert but over the years I’ve come to pick up some tips and tricks to help me produce good data and I thought I’d share: Look […]

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PCR helps to source clinic infection outbreak

Mycobacterium mucogenicum is a bacterium found in soil and water, and it caused an outbreak of bloodstream infections in four young people with sickle cell anaemia at a US University haematology outpatient clinic in 2008. Researchers from East Carolina University used the polymerase chain reaction (PCR) and genotyping to find the source of the outbreak, […]

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Working in real time: A focus on qPCR

There are lots of different types of PCR (polymerase chain reaction), with many different applications, so what’s so special about quantitative PCR (qPCR), also known as real time PCR, RT PCR or qRT-PCR? Measuring in real time Whereas conventional PCR focuses on amplifying DNA, and the results are not seen until the end of the […]

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