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Archive | March, 2013

Normalisation of samples in gene expression analysis

Reverse transcription PCR (RT-PCR) has been used to quantify mRNA (messenger RNA) in gene expression analysis for many years. Researchers generally use the technique to compare two or more samples, for example looking at cancers at different stages, or comparing normal cells and diseased cells, but to control for error in the final results, the […]

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HIV virion

PCR in virus research: Baby’s HIV infection apparently ‘cured’

The polymerase chain reaction (PCR) has played a role in the first ever documented case of a child infected with HIV apparently being ‘cured’.     The child’s mother was HIV positive, though she wasn’t aware of this until she was tested while in labour, and the child, born in Mississippi, was diagnosed with HIV […]

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