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Monitoring Transplant Patients for M. hominis Infections

A recent study has revealed the importance of monitoring transplant donors and recipients for Mycoplasma hominis infections. Mycoplasma hominis colonies M. hominis is a species of bacteria able to infect and penetrate the interior of human cells. It is notoriously difficult to test for and resistant to most antibiotics. In transplant recipients, M. hominis infections can lead to pleurisy, surgical site infection and […]

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Testing Water Samples for Legionella

Evaluation of Legionella qPCR against traditional culture for routine and public health testing of water samples. Legionella is a pathogenic, gram-negative bacteria responsible for causing Legionnaires’ disease, a potentially fatal form of pneumonia, for which there is currently no vaccine. The bacteria are found in water supplies, making the maintenance of water systems essential in preventing the […]

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Successful completion of BJS Biotechnologies projects at UCL

  File 1 – Successful completion of BJS Biotechnologies projects at UCL

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Congratulations

BJS Biotechnologies would like to congratulate our Product Specialist, Charlotte Fenner, for graduating from Imperial College London with a Masters in Applied Bioscience and Biotechnology. The ceremony was held at the Royal Albert Hall last week and was a great celebration to  mark this achievement.

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RT-qPCR to Determine the Role of TEX19 in Cancers

Cancer/testis (CT) genes are one of the major groups of germline genes. They encode proteins known as cancer/testis antigens (CTAs), usually limited to the healthy adult testis. These antigens were initially identified by autologous typing, but thanks to PCR technology it became possible to identify them by expression patterns. Little is understood about the role […]

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injection

World’s First Malaria Vaccine Trial

Ahead of World Malaria Day tomorrow comes an exciting development. The worlds first malaria vaccine has been given the go ahead for large-scale trials in three African countries: Kenya, Ghana and Malawi. These were selected as the initial countries to test the vaccine because they still have high numbers of malaria cases despite prevention programmes already being in […]

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Standard Dilution Series with the xxpress® qPCR Thermal Cycler

The success of a qPCR reaction is evaluated using a standard dilution series, the data from which is used to calculate the reaction efficiency and R2 values. Efficiency can be defined as the increase in amplicon per cycle, whilst R2 is the coefficient of determination. Ideally, the efficiency of a qPCR reaction would be 100%, […]

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Detecting Aquatic Organisms Using eDNA

Nuclear or mitochondrial DNA that is released into the environment from an organism is known as environmental DNA or eDNA. The eDNA of a large number of aquatic organisms can be detected in water samples by qPCR, even when at very low concentrations. This technique is particularly useful when trying to locate rare species that are […]

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The MIQE Guidlines

The Minimum Information for Publication of Quantitative Real-Time PCR Experiments. The MIQE guidelines were introduced to reduce the lack of standardisation in performing and evaluating qPCR experiments. Previously, a lack of consensus was causing several issues within the field. In particular, the publication of insufficient details presented problems when critically evaluating an experiment or attempting […]

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Figure 3 Thermal image of plate showing heated areas

Thermal Uniformity with the xxpress® qPCR Thermal Cycler

qPCR is a molecular biology technique that utilises temperature cycles to amplify and quantify DNA. Thermal uniformity is essential when performing qPCR to ensure repeatable, reliable results. Undershoots can prevent reaction steps from reaching completion, whilst overshoots can cause the sample to degrade.  At BJS Biotechnologies we have developed the xxpress® qPCR thermal cycler which […]

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