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Fundamentals of PCR

The MIQE Guidlines

The Minimum Information for Publication of Quantitative Real-Time PCR Experiments. The MIQE guidelines were introduced to reduce the lack of standardisation in performing and evaluating qPCR experiments. Previously, a lack of consensus was causing several issues within the field. In particular, the publication of insufficient details presented problems when critically evaluating an experiment or attempting […]

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The Story of the Polymerase Chain Reaction

 A recent article, by Maggie Koreth-Baker for Nautilus, details the story of the Polymerase Chain Reaction “from a Pink Squiggle to the Human Genome Project”. The article details the discovery of the thermophilic bacterium, Thermus Aquaticus, from which heat-resistant Taq DNA polymerase was obtained. “This Yellowstone bacterium sparked PCR, one of the great advances in genetics.” […]

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Celebrating PCR at 30

In 2013, the polymerase chain reaction (PCR) turned 30. To celebrate these, here’s a roundup of articles celebrating PCR on the web and from our archive. Start with PCR 101: An introduction to PCR, and then read PCR Turns 30, and find out more about the history of PCR. Learn about PCR, From the Past […]

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MIQE PCR smartphone app

MIQE app for PCR – bringing PCR tools to your smartphone

These days, few people are seen without a smartphone, whether it’s an Android or an iPhone, and their computing power and storage capabilities are increasing. The numbers of apps for smartphones are increasing, and these are starting to make their way into the labs, for scanning information, capturing images, storing data, cutting the amount of […]

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PCR, from the past to the future

The polymerase chain reaction (PCR), sometimes described as ‘molecular photocopying’, is a laboratory technique that allows researchers and technicians to replicate and amplify DNA or RNA from small or damaged samples, even down to a single strand. The technology was first created in 1983 by biochemist Kary Mullis, who was working for the biotechnology company […]

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The role of PCR in single cell analysis

The polymerase chain reaction (PCR) has a wealth of applications in research, but perhaps one of its most challenging roles in single cell analysis, amplifying the DNA from just a single cell, or a very small sample of cells.   Real-time single cell PCR can be used in gene expression analysis. This has a role […]

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PCR in mutation analysis

Mutation analysis is the process of finding known and unknown mutations in DNA and RNA, and PCR (polymerase chain reaction) is used as part of the method. In the clinic, mutation analysis can be used to detect known mutations for disease diagnosis or select the patients who are most likely to respond in personalised medicine. […]

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Happy DNA Day: Celebrate the 60th anniversary of the discovery of the structure of DNA

Happy 60th birthday to DNA on 23 April 2013, DNA Day! Today marks the 60th anniversary of the publication of papers in Nature on the discovery of the structure of DNA in 1953. A lot has happened since the publication of the three groundbreaking papers authored by Watson & Crick, Franklin and Gosling, and Wilkins, […]

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Normalisation of samples in gene expression analysis

Reverse transcription PCR (RT-PCR) has been used to quantify mRNA (messenger RNA) in gene expression analysis for many years. Researchers generally use the technique to compare two or more samples, for example looking at cancers at different stages, or comparing normal cells and diseased cells, but to control for error in the final results, the […]

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Applications in PCR: Using PCR to track migration

PCR (polymerase chain reaction) amplification of ancient and modern DNA samples can be used by researchers to track migration of animal and human populations, shedding light on evolution and the movement of ethnic groups across continents. There are many different studies that have used this technique, and this post includes a few different examples. Tracking […]

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