Sort by:

PCR in diagnosis

Monitoring Transplant Patients for M. hominis Infections

A recent study has revealed the importance of monitoring transplant donors and recipients for Mycoplasma hominis infections. Mycoplasma hominis colonies M. hominis is a species of bacteria able to infect and penetrate the interior of human cells. It is notoriously difficult to test for and resistant to most antibiotics. In transplant recipients, M. hominis infections can lead to pleurisy, surgical site infection and […]

read more

HRM for the Detection of Important Poxviruses

A high resolution melt (HRM) is a powerful, post-PCR, analysis technique used to identify the DNA products present. First of all PCR is performed with an intercalating dye present. These dyes have the unique property of only binding to double-stranded DNA. Once incorporated into the newly synthesised amplicon the dye will fluoresce brightly. Performing a high-resolution […]

read more

New Zika test approved for CDC use

Zika, Dengue and Chikungunya; 3 viruses alike in their symptoms and their transmission by Aedes mosquitoes. Former PCR-based Zika tests were unable to rule out infections with Dengue or Chikungunya. A trioplex real-time RT-PCR assay has now been developed for the detection and differentiation of RNA from these three viruses. This qPCR assay has been approved […]

read more

PCR a valuable tool for diagnosing HIV infections

The World Health Organisation estimates that approximately 36.7 million people are living with HIV globally. This epidemic still poses serious public health issues. Diagnosis is a key factor in helping to prevent the disease spread as well as ensuring that patients receive the correct treatment as soon as possible. The polymerase chain reaction is used […]

read more

PCR to determine the likelihood of relapse in AML patients

Acute Myeloid Leukemia (AML) is a rare form of cancer affecting the Myeloid cells in the blood. It most commonly results from a mutation in the FMS-related Tyrosine Kinase 3 (FLT3) gene. The current treatment for AML is Allogenic Stem Cell Transplantation; the transfer of stem cells from a healthy donor to the patient following chemotherapy or […]

read more

A new PCR primer database for RNA virus detection

A new database has been created which outlines primers for use in the polymerase chain reaction (PCR) to detect RNA viruses. Details of this exciting new database, MRPrimerV, were published in Nucleic Acids Research.   “Abstract: Many infectious diseases are caused by viral infections, and in particular by RNA viruses such as MERS, Ebola and Zika. To understand […]

read more
DNA double helix

PCR helps to create potential myeloma blood test

Researchers from the NIH, the National Cancer Institute (NCI), and the Dana-Farber Cancer Institute of Harvard Medical School have used the polymerase chain reaction (PCR) to help to create a potential multiple myeloma diagnostic. The results were published in the Journal of Molecular Diagnostics. Multiple myeloma is a cancer of the bone marrow with a […]

read more
Lung adenocarcinoma

DNA alterations could be precursors of lung cancer

The polymerase chain reaction has helped a team of US researchers to see the very earliest genetic changes that could mark the onset of the most common form of lung cancer. The results are published online in Nature Communications. Understanding the precursors to lung cancer could improve the diagnosis and treatment of this form of […]

read more
DNA spiral cartoon

Biomarker could predict gastric cancer survival

Chinese researchers have found an RNA biomarker that could predict survival for patients with gastric cancer, according to a paper published in the American Journal of Pathology. Gastric cancer is one of the most common cancers in developing countries. It is often diagnosed at a late stage, and has a high death rate. The aim […]

read more
Chromosome 7

New strategy uses PCR to improve detection of mutations in hereditary colorectal cancer

Using next generation genetic techniques and PCR could help doctors identify people with Lynch syndrome caused by a mutation in the PMS2 gene. The results are published in the Journal of Molecular Diagnostics. Lynch syndrome (also known as hereditary non-polyposis colorectal cancer or HNPCC), is usually caused by heterozygous germline mutations in the DNA mismatch […]

read more