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PCR in research

Monitoring Transplant Patients for M. hominis Infections

A recent study has revealed the importance of monitoring transplant donors and recipients for Mycoplasma hominis infections. Mycoplasma hominis colonies M. hominis is a species of bacteria able to infect and penetrate the interior of human cells. It is notoriously difficult to test for and resistant to most antibiotics. In transplant recipients, M. hominis infections can lead to pleurisy, surgical site infection and […]

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Successful completion of BJS Biotechnologies projects at UCL

  File 1 – Successful completion of BJS Biotechnologies projects at UCL

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RT-qPCR to Determine the Role of TEX19 in Cancers

Cancer/testis (CT) genes are one of the major groups of germline genes. They encode proteins known as cancer/testis antigens (CTAs), usually limited to the healthy adult testis. These antigens were initially identified by autologous typing, but thanks to PCR technology it became possible to identify them by expression patterns. Little is understood about the role […]

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injection

World’s First Malaria Vaccine Trial

Ahead of World Malaria Day tomorrow comes an exciting development. The worlds first malaria vaccine has been given the go ahead for large-scale trials in three African countries: Kenya, Ghana and Malawi. These were selected as the initial countries to test the vaccine because they still have high numbers of malaria cases despite prevention programmes already being in […]

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Detecting Aquatic Organisms Using eDNA

Nuclear or mitochondrial DNA that is released into the environment from an organism is known as environmental DNA or eDNA. The eDNA of a large number of aquatic organisms can be detected in water samples by qPCR, even when at very low concentrations. This technique is particularly useful when trying to locate rare species that are […]

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HRM for the Detection of Important Poxviruses

A high resolution melt (HRM) is a powerful, post-PCR, analysis technique used to identify the DNA products present. First of all PCR is performed with an intercalating dye present. These dyes have the unique property of only binding to double-stranded DNA. Once incorporated into the newly synthesised amplicon the dye will fluoresce brightly. Performing a high-resolution […]

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PCR a valuable tool for diagnosing HIV infections

The World Health Organisation estimates that approximately 36.7 million people are living with HIV globally. This epidemic still poses serious public health issues. Diagnosis is a key factor in helping to prevent the disease spread as well as ensuring that patients receive the correct treatment as soon as possible. The polymerase chain reaction is used […]

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PCR to determine the likelihood of relapse in AML patients

Acute Myeloid Leukemia (AML) is a rare form of cancer affecting the Myeloid cells in the blood. It most commonly results from a mutation in the FMS-related Tyrosine Kinase 3 (FLT3) gene. The current treatment for AML is Allogenic Stem Cell Transplantation; the transfer of stem cells from a healthy donor to the patient following chemotherapy or […]

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A new PCR primer database for RNA virus detection

A new database has been created which outlines primers for use in the polymerase chain reaction (PCR) to detect RNA viruses. Details of this exciting new database, MRPrimerV, were published in Nucleic Acids Research.   “Abstract: Many infectious diseases are caused by viral infections, and in particular by RNA viruses such as MERS, Ebola and Zika. To understand […]

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DNA spiral cartoon

Biomarker could predict gastric cancer survival

Chinese researchers have found an RNA biomarker that could predict survival for patients with gastric cancer, according to a paper published in the American Journal of Pathology. Gastric cancer is one of the most common cancers in developing countries. It is often diagnosed at a late stage, and has a high death rate. The aim […]

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