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PCR news

Monitoring Transplant Patients for M. hominis Infections

A recent study has revealed the importance of monitoring transplant donors and recipients for Mycoplasma hominis infections. Mycoplasma hominis colonies M. hominis is a species of bacteria able to infect and penetrate the interior of human cells. It is notoriously difficult to test for and resistant to most antibiotics. In transplant recipients, M. hominis infections can lead to pleurisy, surgical site infection and […]

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Testing Water Samples for Legionella

Evaluation of Legionella qPCR against traditional culture for routine and public health testing of water samples. Legionella is a pathogenic, gram-negative bacteria responsible for causing Legionnaires’ disease, a potentially fatal form of pneumonia, for which there is currently no vaccine. The bacteria are found in water supplies, making the maintenance of water systems essential in preventing the […]

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Successful completion of BJS Biotechnologies projects at UCL

  File 1 – Successful completion of BJS Biotechnologies projects at UCL

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Detecting Aquatic Organisms Using eDNA

Nuclear or mitochondrial DNA that is released into the environment from an organism is known as environmental DNA or eDNA. The eDNA of a large number of aquatic organisms can be detected in water samples by qPCR, even when at very low concentrations. This technique is particularly useful when trying to locate rare species that are […]

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Design of a Synthetic Yeast Genome

A multidisciplinary team at The Department of Biomedical Engineering, Johns Hopkins University, have designed a synthetic eukaryotic genome. The project, known as Sc2.0, resulted in a highly modified Saccharomyces cerevisiae genome which was ~8% smaller, with 1.1 megabases inserted, deleted or altered.   An open-source framework, called BioStudio, was used to design the genome. It is hoped […]

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HRM for the Detection of Important Poxviruses

A high resolution melt (HRM) is a powerful, post-PCR, analysis technique used to identify the DNA products present. First of all PCR is performed with an intercalating dye present. These dyes have the unique property of only binding to double-stranded DNA. Once incorporated into the newly synthesised amplicon the dye will fluoresce brightly. Performing a high-resolution […]

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New Zika test approved for CDC use

Zika, Dengue and Chikungunya; 3 viruses alike in their symptoms and their transmission by Aedes mosquitoes. Former PCR-based Zika tests were unable to rule out infections with Dengue or Chikungunya. A trioplex real-time RT-PCR assay has now been developed for the detection and differentiation of RNA from these three viruses. This qPCR assay has been approved […]

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PCR a valuable tool for diagnosing HIV infections

The World Health Organisation estimates that approximately 36.7 million people are living with HIV globally. This epidemic still poses serious public health issues. Diagnosis is a key factor in helping to prevent the disease spread as well as ensuring that patients receive the correct treatment as soon as possible. The polymerase chain reaction is used […]

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PCR to determine the likelihood of relapse in AML patients

Acute Myeloid Leukemia (AML) is a rare form of cancer affecting the Myeloid cells in the blood. It most commonly results from a mutation in the FMS-related Tyrosine Kinase 3 (FLT3) gene. The current treatment for AML is Allogenic Stem Cell Transplantation; the transfer of stem cells from a healthy donor to the patient following chemotherapy or […]

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A new PCR primer database for RNA virus detection

A new database has been created which outlines primers for use in the polymerase chain reaction (PCR) to detect RNA viruses. Details of this exciting new database, MRPrimerV, were published in Nucleic Acids Research.   “Abstract: Many infectious diseases are caused by viral infections, and in particular by RNA viruses such as MERS, Ebola and Zika. To understand […]

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