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Real time PCR

Testing Water Samples for Legionella

Evaluation of Legionella qPCR against traditional culture for routine and public health testing of water samples. Legionella is a pathogenic, gram-negative bacteria responsible for causing Legionnaires’ disease, a potentially fatal form of pneumonia, for which there is currently no vaccine. The bacteria are found in water supplies, making the maintenance of water systems essential in preventing the […]

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RT-qPCR to Determine the Role of TEX19 in Cancers

Cancer/testis (CT) genes are one of the major groups of germline genes. They encode proteins known as cancer/testis antigens (CTAs), usually limited to the healthy adult testis. These antigens were initially identified by autologous typing, but thanks to PCR technology it became possible to identify them by expression patterns. Little is understood about the role […]

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Standard Dilution Series with the xxpress® qPCR Thermal Cycler

The success of a qPCR reaction is evaluated using a standard dilution series, the data from which is used to calculate the reaction efficiency and R2 values. Efficiency can be defined as the increase in amplicon per cycle, whilst R2 is the coefficient of determination. Ideally, the efficiency of a qPCR reaction would be 100%, […]

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Detecting Aquatic Organisms Using eDNA

Nuclear or mitochondrial DNA that is released into the environment from an organism is known as environmental DNA or eDNA. The eDNA of a large number of aquatic organisms can be detected in water samples by qPCR, even when at very low concentrations. This technique is particularly useful when trying to locate rare species that are […]

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The MIQE Guidlines

The Minimum Information for Publication of Quantitative Real-Time PCR Experiments. The MIQE guidelines were introduced to reduce the lack of standardisation in performing and evaluating qPCR experiments. Previously, a lack of consensus was causing several issues within the field. In particular, the publication of insufficient details presented problems when critically evaluating an experiment or attempting […]

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New Zika test approved for CDC use

Zika, Dengue and Chikungunya; 3 viruses alike in their symptoms and their transmission by Aedes mosquitoes. Former PCR-based Zika tests were unable to rule out infections with Dengue or Chikungunya. A trioplex real-time RT-PCR assay has now been developed for the detection and differentiation of RNA from these three viruses. This qPCR assay has been approved […]

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PCR a valuable tool for diagnosing HIV infections

The World Health Organisation estimates that approximately 36.7 million people are living with HIV globally. This epidemic still poses serious public health issues. Diagnosis is a key factor in helping to prevent the disease spread as well as ensuring that patients receive the correct treatment as soon as possible. The polymerase chain reaction is used […]

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Introducing SEPSIS-xxpress

SEPSIS-xxpress is BJS Biotechnologies’ rapid POC (point of care) sepsis testing system in development as part of a London-based consortium. The new system combines BJS’s experience in sample preparation, automation and real-time PCR with Brunel University London’s microfluidic engineering skills, while St Georges, University of London, will run clinical trials. With an estimated 25 million annual […]

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BJS Biotechnologies to lead £5m London consortium developing rapid sepsis test

Leading life science instrumentation company BJS Biotechnologies has announced it is to lead a London consortium to finalise development of a rapid POC (point of care) sepsis testing system. Called SEPSIS-xxpress, the new system combines BJS’s excellence in sample preparation, automation and real-time PCR with Brunel University London’s microfluidic engineering skills, while St Georges, University of […]

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PCR-based diagnostics could help tuberculosis eradication

Tuberculosis is a widespread bacterial disease, and while it is treatable, its eradication is being held up by the problems with finding those people who have latent infection. Researchers at Yonsei University, Republic of Korea, have created a multiple-target, real-time RT-PCR (reverse transcription polymerase chain reaction) assay that can distinguish between active disease and latent […]

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