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White papers

Standard Dilution Series with the xxpress® qPCR Thermal Cycler

The success of a qPCR reaction is evaluated using a standard dilution series, the data from which is used to calculate the reaction efficiency and R2 values. Efficiency can be defined as the increase in amplicon per cycle, whilst R2 is the coefficient of determination. Ideally, the efficiency of a qPCR reaction would be 100%, […]

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Figure 3 Thermal image of plate showing heated areas

Thermal Uniformity with the xxpress® qPCR Thermal Cycler

qPCR is a molecular biology technique that utilises temperature cycles to amplify and quantify DNA. Thermal uniformity is essential when performing qPCR to ensure repeatable, reliable results. Undershoots can prevent reaction steps from reaching completion, whilst overshoots can cause the sample to degrade.  At BJS Biotechnologies we have developed the xxpress® qPCR thermal cycler which […]

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PCR, from the past to the future

The polymerase chain reaction (PCR), sometimes described as ‘molecular photocopying’, is a laboratory technique that allows researchers and technicians to replicate and amplify DNA or RNA from small or damaged samples, even down to a single strand. The technology was first created in 1983 by biochemist Kary Mullis, who was working for the biotechnology company […]

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