PCR a valuable tool for diagnosing HIV infections

The World Health Organisation estimates that approximately 36.7 million people are living with HIV globally. This epidemic still poses serious public health issues. Diagnosis is a key factor in helping to prevent the disease spread as well as ensuring that patients receive the correct treatment as soon as possible.

The polymerase chain reaction is used to diagnose very early HIV infections, before antibodies have even been produced. Viral RNA can be detected using primers unique to the virus’s genome.  This method is also able to accurately quantify the amount of viral RNA present in the sample.

However, until recently it was not possible to accurately detect HIV-1 and HIV-2 RNA in parallel. A recently published article has described a highly sensitive and accurate qPCR assay to enable parallel detection. This could be used as an important method to differentiate between HIV-1 and HIV-2 infections in the future.

Article Summary:

“Because western blotting occasionally causes cross-reactions between human immunodeficiency virus (HIV)-1 and HIV-2, it is difficult to distinguish a coinfection status from a false-positive result. Therefore, we developed a qualitative real-time PCR assay to detect HIV-1 and HIV-2 RNA that can be performed in parallel. Viral RNA extracted from 500 ml of plasma was examined using real-time PCR with minor groove binder probes. Bovine leukemia virus was used as an internal standard. The sensitivity was determined by probit regression analysis using the World Health Organization international standards for HIV-1 and HIV-2. The lower detection limits at a 95z hit rate were 54 IU/ml for HIV-1 and 5.0 IU/ml for HIV-2, which were lower than any HIV-2 assays reported previously. HIV-1 RNA was detected in 51 of 52 HIV-1 seropositive plasma samples. HIV-2 RNA was detected in 7 of 10 HIV-2 seropositive plasma samples. Non-specific signals and cross reactivity between HIV-1 and HIV-2 were not observed in 100 HIV seronegative samples. The assay developed in this study is highly sensitive and specific for the detection of HIV-1 and HIV-2 RNA. The test is expected to be useful for the differential diagnosis of HIV-1 and HIV-2 infections. ”

 

 

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